Altered Selectivity in Glutamate Transporter Mutant [Neurobiology]
In this study, we characterize mutants of this residue from the neuronal transporter EAAC1, Asn-451. None of the mutants, except for N451S, were able to exhibit transport. However, the Km of this mutant for l-aspartate was increased ∼30-fold. Remarkably, the increase for d-aspartate and l-glutamate was 250- and 400-fold, respectively. Moreover, the cation specificity of N451S was altered because sodium but not lithium could support transport. A similar change in cation specificity was observed with a mutant of a conserved threonine residue, T370S, also implicated to participate in the novel Na+ site together with the bound substrate. In further contrast to the wild type transporter, only l-aspartate was able to activate the uncoupled anion conductance by N451S, but with an almost 1000-fo...

COUMADIN (Warfarin Sodium) Tablet [Cardinal Health]
Updated Date: May 17, 2012 EST (Source: DailyMed Drug Label Updates for the last seven days (since May 20, 2007 EST))