Application of an In-Cell Western assay for measurement of influenza A virus replication.
Authors: Wan Y, Zhou Z, Yang Y, Wang J, Hung T
Influenza A pandemics present enormous challenges to modern medicine. To control such pandemics, quantitative assays characterised by rapidity, high sensitivity, and high-throughput are critical in determining the susceptibility of the influenza A virus to antiviral drugs and for screening chemicals that can inhibit viral replication effectively. In the present study, a rapid and quantitative method to determine influenza A virus replication was developed by an In-Cell Western (ICW) assay. This assay was found to be useful for monitoring the kinetics of influenza A virus replication, as viral nucleoprotein production could be correlated to both increasing doses of viral infection and to the lapse of time during viral infection. Compared to...
Beneficial Effect of Amantadine on Postoperative Pain Reduction and Consumption of Morphine in Patients Subjected to Elective Spine Surgery
Conclusions. Pre‐ and postoperative administration of amantadine significantly reduced fentanyl use during operation, as well as reduced the postoperative pain and decreased morphine consumption in young patients undergoing orthopedic surgery. (Source: Pain Medicine)
