In vitro evaluation of a mammary gland specific expression vector encoding recombinant human lysozyme for development of transgenic dairy goat embryos.
Authors: Gui T, Zhang M, Chen J, Zhang Y, Zhou N, Zhang Y, Tao J, Sui L, Li Y, Liu Y, Zhang X, Zhang Y
Abstract
A vector expressing human lysozyme (pBC1-hLYZ-GFP-Neo) was evaluated for gene and protein expression following liposome-mediated transformation of C-127 mouse mammary cancer cells. Cultures of G418-resistant clones were harvested 24-72 h after induction with prolactin, insulin and hydrocortisone. Target gene expression was analyzed by RT-PCR and Western blot and recombinant human lysozyme (rhLYZ) bacteriostatic activity was also evaluated. The hLYZ gene was correctly transcribed and translated in C-127 cells and hLYZ inhibited gram-positive bacterial growth, indicating the potential of this expression vector for development of a mammary gland bioreactor in goats. Guanzho...
Sudan: Residents Angry At MSF Blockade in Jebel Si
[Radio Dabanga]
Jebel Si -
Sheikhs (native leaders) and displaced people have rejected the Sudanese government blocking the humanitarian organisation Doctors Without Borders (MSF) from working in Jebel Si. (Source: AllAfrica News: Health and Medicine)
