Vimentin-mediated signaling is required for IbeA{+} E. coli K1 invasion of human brain microvascular endothelial cells
In this report, we demonstrated that vimentin is a primary receptor required for IbeA#x002B; E. coli K1-induced signaling and invasion of HBMEC, based on the following observations. First, E44 (IbeA#x002B; E. coli K1 strain) invasion was blocked by vimentin inhibitors (Withfferin A and Acrylamide), a recombinant protein containing vimentin head domain and an antibody against the head domain, respectively. Secondly, overexpression of GFP-vimentin and GFP-vimentin head domain deletion mutant (VDM) significantly increased and decreased bacterial invasion, respectively. Thirdly, bacterial invasion was positively correlated with phosphorylation of vimentin at serine 82 by calcium calmodulin-dependent kinase II (CaM kinase II) and IbeA#x002B; E. coli-induced phosphorylation of extracellular r...
Komen Foundation VP resigns after funding controversy
Karen Handel, a vice president with the Susan G. Komen for the Cure Foundation, resigned her position Tuesday following a controversy over funding for Planned Parenthood projects, the foundation said. (Source: CNN.com - Health)
