Bread and Other Common Foods Top List of Sodium Culprits
About 90% of us get too much sodium every day, and most of it comes from common restaurant or grocery store items, the CDC reports. The No. 1 culprit? Bread and rolls. (Source: WebMD Health)div id=medwormpbiMedWorm Message:/i/b Register forba href=http://www.medmatcha.com target =_selfMedMatcha, MedWorm's medical advertising network/a/b, and receive $5 free advertising./p/div
Rapid and efficient proteolysis for proteomic analysis by protease-immobilized microreactor
In this study, trypsin- or [alpha]-chymotrypsin-immobilized microreactors prepared by a microfluidics-based enzyme-immobilization technique were studied for rapid sample preparation in proteomic analysis. The kinetic studies for hydrolysis of substrate by microreactors revealed that immobilized proteases had higher hydrolytic efficiency than those performed by in-solution digestion. The performance of the microreactors was evaluated by digesting cytochrome c and BSA. Protein digestion was achieved within a short period of time ([sim]5 min) at 30°C without any complicated reduction and alkylation procedures. The efficiency of digestion by trypsin-immobilized reactor was evaluated by analyzing the sequence coverage, which was 47 and 12% for cytochrome c and BSA, respectively. These values w...
