Simultaneous analysis of six cardiovascular drugs by capillary electrophoresis coupled with electrochemical and electrochemiluminescence detection, using a chemometrical optimization approach
AbstractCE coupled with dual electrochemical (EC) and electrochemiluminescence (ECL) detection was optimized for simultaneous analysis of six cardiovascular drugs (alprenolol, propafenone, acebutolol, verapamil, atenolol and metoprolol) via central composite design. Following this study, three critical electrophoretic factors governing the CE separation were investigated: TrisâH3PO4 buffer concentration, buffer pH value and separation voltage. A modified chromatographic response was adopted for evaluating CE separation quality. Optimum conditions were achieved using TrisâH3PO4 buffer 35.6âmM (pH 2.3) separated at 13.9âkV, which was employed experimentally and led to the successful simultaneous separation of the above six drugs. The good agreement of the chromatographic response was...
Pharmacological Basis for the Medicinal Use of Lepidium sativum in Airways Disorders
Lepidium sativum is widely used in folk medicine for treatment of hyperactive airways disorders, such as asthma, bronchitis and cough. The crude extract of Lepidium sativum (Ls.Cr) inhibited carbachol (CCh, 1#x2009;#x03BC;M-) and K+ (80#x2009;mM-) induced contractions in a pattern similar to that of dicyclomine. Ls.Cr at 0.03#x2009;mg/mL produced a rightward parallel shift of CCh curves, followed by nonparallel shift at higher concentration (0.1#x2009;mg/mL), suppressing maximum response, similar to that caused by dicyclomine. Pretreatment of tissues with Ls.Cr (0.1#8211;0.3#x2009;mg/mL) shifted Ca++ concentration-response curves (CRCs) to right, as produced by verapamil. Ls.Cr at low concentrations (0.03#8211;0.1#x2009;mg/mL) caused leftward shift of isoprenaline-induced inhibito...
